Bioethanol production from hemicellulose rich Populus nigra involving recombinant hemicellulases from Clostridium thermocellum.
Identifieur interne : 002347 ( Main/Exploration ); précédent : 002346; suivant : 002348Bioethanol production from hemicellulose rich Populus nigra involving recombinant hemicellulases from Clostridium thermocellum.
Auteurs : Ashutosh Gupta [Inde] ; Saprativ P. Das [Inde] ; Arabinda Ghosh [Inde] ; Rajan Choudhary [Inde] ; Debasish Das [Inde] ; Arun Goyal [Inde]Source :
- Bioresource technology [ 1873-2976 ] ; 2014.
Descripteurs français
- KwdFr :
- Ammoniac (composition chimique), Biocarburants (MeSH), Biomasse (MeSH), Bioréacteurs (microbiologie), Biotechnologie (méthodes), Cellulose (MeSH), Chromatographie d'échange d'ions (MeSH), Chromatographie sur couche mince (MeSH), Clostridium thermocellum (enzymologie), Escherichia coli (enzymologie), Fermentation (MeSH), Feuilles de plante (métabolisme), Feuilles de plante (ultrastructure), Glycosidases (métabolisme), Hydrolyse (MeSH), Lignine (MeSH), Normes de référence (MeSH), Polyosides (métabolisme), Populus (métabolisme), Protéines recombinantes (métabolisme), Spectroscopie infrarouge à transformée de Fourier (MeSH), Techniques de culture cellulaire en batch (MeSH), Éthanol (métabolisme).
- MESH :
- composition chimique : Ammoniac.
- enzymologie : Clostridium thermocellum, Escherichia coli.
- microbiologie : Bioréacteurs.
- métabolisme : Feuilles de plante, Glycosidases, Polyosides, Populus, Protéines recombinantes, Éthanol.
- méthodes : Biotechnologie.
- ultrastructure : Biocarburants, Biomasse, Cellulose, Chromatographie d'échange d'ions, Chromatographie sur couche mince, Fermentation, Feuilles de plante, Hydrolyse, Lignine, Normes de référence, Spectroscopie infrarouge à transformée de Fourier, Techniques de culture cellulaire en batch.
English descriptors
- KwdEn :
- Ammonia (chemistry), Batch Cell Culture Techniques (MeSH), Biofuels (MeSH), Biomass (MeSH), Bioreactors (microbiology), Biotechnology (methods), Cellulose (MeSH), Chromatography, Ion Exchange (MeSH), Chromatography, Thin Layer (MeSH), Clostridium thermocellum (enzymology), Escherichia coli (enzymology), Ethanol (metabolism), Fermentation (MeSH), Glycoside Hydrolases (metabolism), Hydrolysis (MeSH), Lignin (MeSH), Plant Leaves (metabolism), Plant Leaves (ultrastructure), Polysaccharides (metabolism), Populus (metabolism), Recombinant Proteins (metabolism), Reference Standards (MeSH), Spectroscopy, Fourier Transform Infrared (MeSH).
- MESH :
- chemical , chemistry : Ammonia.
- enzymology : Clostridium thermocellum, Escherichia coli.
- chemical , metabolism : Ethanol, Glycoside Hydrolases, Plant Leaves, Polysaccharides, Populus, Recombinant Proteins.
- methods : Biotechnology.
- microbiology : Bioreactors.
- ultrastructure : Plant Leaves.
- Batch Cell Culture Techniques, Biofuels, Biomass, Cellulose, Chromatography, Ion Exchange, Chromatography, Thin Layer, Fermentation, Hydrolysis, Lignin, Reference Standards, Spectroscopy, Fourier Transform Infrared.
Abstract
Bioethanol was produced from poplar leafy biomass rich in hemicelluloses content involving recombinant Clostridium thermocellum hemicellulases and pentose sugar utilizing Candida shehatae. FT-IR analysis revealed effective AFEX pretreatment of poplar leaves. Repetitive batch strategy yielded ∼1.5-fold rise in cell biomass and specific activity of both, acetylxylanesterase (Axe) and GH43 hemicellulase. TLC and HPAEC exhibited xylose and arabinose release from hydrolyzed biomass. SSF trial with 1% (wv(-1)) pretreated poplar and mixed enzymes showed ∼1.5-fold higher ethanol titre as compared with SHF. The shake flask SSF with 5% (wv(-1)) pretreated poplar furnished 4.56 and 5.43gL(-1) ethanol with Axe and mixed enzymes, respectively. Whereas, bioreactor scale-up exhibited ∼1.25-fold increase in ethanol titres (5.68, 6.75gL(-1)) as compared with shake flask with an yield of 0.295 (gg(-1)) and 0.351 (gg(-1)), respectively with Axe and mixed enzymes.
DOI: 10.1016/j.biortech.2014.03.132
PubMed: 24767793
Affiliations:
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Electronic address: arungoyl@iitg.ernet.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam</wicri:regionArea><wicri:noRegion>Assam</wicri:noRegion></affiliation></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ammonia (chemistry)</term><term>Batch Cell Culture Techniques (MeSH)</term><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>Bioreactors (microbiology)</term><term>Biotechnology (methods)</term><term>Cellulose (MeSH)</term><term>Chromatography, Ion Exchange (MeSH)</term><term>Chromatography, Thin Layer (MeSH)</term><term>Clostridium thermocellum (enzymology)</term><term>Escherichia coli (enzymology)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Glycoside Hydrolases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Lignin (MeSH)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (ultrastructure)</term><term>Polysaccharides (metabolism)</term><term>Populus (metabolism)</term><term>Recombinant Proteins (metabolism)</term><term>Reference Standards (MeSH)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ammoniac (composition chimique)</term><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Bioréacteurs (microbiologie)</term><term>Biotechnologie (méthodes)</term><term>Cellulose (MeSH)</term><term>Chromatographie d'échange d'ions (MeSH)</term><term>Chromatographie sur couche mince (MeSH)</term><term>Clostridium thermocellum (enzymologie)</term><term>Escherichia coli (enzymologie)</term><term>Fermentation (MeSH)</term><term>Feuilles de plante (métabolisme)</term><term>Feuilles de plante (ultrastructure)</term><term>Glycosidases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Lignine (MeSH)</term><term>Normes de référence (MeSH)</term><term>Polyosides (métabolisme)</term><term>Populus (métabolisme)</term><term>Protéines recombinantes (métabolisme)</term><term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term><term>Techniques de culture cellulaire en batch (MeSH)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ammonia</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Ammoniac</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Clostridium thermocellum</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Clostridium thermocellum</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term><term>Glycoside Hydrolases</term><term>Plant Leaves</term><term>Polysaccharides</term><term>Populus</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bioréacteurs</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bioreactors</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Glycosidases</term><term>Polyosides</term><term>Populus</term><term>Protéines recombinantes</term><term>Éthanol</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Batch Cell Culture Techniques</term><term>Biofuels</term><term>Biomass</term><term>Cellulose</term><term>Chromatography, Ion Exchange</term><term>Chromatography, Thin Layer</term><term>Fermentation</term><term>Hydrolysis</term><term>Lignin</term><term>Reference Standards</term><term>Spectroscopy, Fourier Transform Infrared</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Biocarburants</term><term>Biomasse</term><term>Cellulose</term><term>Chromatographie d'échange d'ions</term><term>Chromatographie sur couche mince</term><term>Fermentation</term><term>Feuilles de plante</term><term>Hydrolyse</term><term>Lignine</term><term>Normes de référence</term><term>Spectroscopie infrarouge à transformée de Fourier</term><term>Techniques de culture cellulaire en batch</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bioethanol was produced from poplar leafy biomass rich in hemicelluloses content involving recombinant Clostridium thermocellum hemicellulases and pentose sugar utilizing Candida shehatae. FT-IR analysis revealed effective AFEX pretreatment of poplar leaves. Repetitive batch strategy yielded ∼1.5-fold rise in cell biomass and specific activity of both, acetylxylanesterase (Axe) and GH43 hemicellulase. TLC and HPAEC exhibited xylose and arabinose release from hydrolyzed biomass. SSF trial with 1% (wv(-1)) pretreated poplar and mixed enzymes showed ∼1.5-fold higher ethanol titre as compared with SHF. The shake flask SSF with 5% (wv(-1)) pretreated poplar furnished 4.56 and 5.43gL(-1) ethanol with Axe and mixed enzymes, respectively. Whereas, bioreactor scale-up exhibited ∼1.25-fold increase in ethanol titres (5.68, 6.75gL(-1)) as compared with shake flask with an yield of 0.295 (gg(-1)) and 0.351 (gg(-1)), respectively with Axe and mixed enzymes. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24767793</PMID><DateCompleted><Year>2015</Year><Month>01</Month><Day>27</Day></DateCompleted><DateRevised><Year>2014</Year><Month>06</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>165</Volume><PubDate><Year>2014</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Bioethanol production from hemicellulose rich Populus nigra involving recombinant hemicellulases from Clostridium thermocellum.</ArticleTitle><Pagination><MedlinePgn>205-13</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2014.03.132</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(14)00437-4</ELocationID><Abstract><AbstractText>Bioethanol was produced from poplar leafy biomass rich in hemicelluloses content involving recombinant Clostridium thermocellum hemicellulases and pentose sugar utilizing Candida shehatae. FT-IR analysis revealed effective AFEX pretreatment of poplar leaves. Repetitive batch strategy yielded ∼1.5-fold rise in cell biomass and specific activity of both, acetylxylanesterase (Axe) and GH43 hemicellulase. TLC and HPAEC exhibited xylose and arabinose release from hydrolyzed biomass. SSF trial with 1% (wv(-1)) pretreated poplar and mixed enzymes showed ∼1.5-fold higher ethanol titre as compared with SHF. The shake flask SSF with 5% (wv(-1)) pretreated poplar furnished 4.56 and 5.43gL(-1) ethanol with Axe and mixed enzymes, respectively. Whereas, bioreactor scale-up exhibited ∼1.25-fold increase in ethanol titres (5.68, 6.75gL(-1)) as compared with shake flask with an yield of 0.295 (gg(-1)) and 0.351 (gg(-1)), respectively with Axe and mixed enzymes. </AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Ashutosh</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Saprativ P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ghosh</LastName><ForeName>Arabinda</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choudhary</LastName><ForeName>Rajan</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713209, West Bengal, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Debasish</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goyal</LastName><ForeName>Arun</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: arungoyl@iitg.ernet.in.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>04</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>7664-41-7</RegistryNumber><NameOfSubstance UI="D000641">Ammonia</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="C023305">hemicellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000641" MajorTopicYN="N">Ammonia</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D061249" MajorTopicYN="N">Batch Cell Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="Y">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019149" MajorTopicYN="N">Bioreactors</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002852" MajorTopicYN="N">Chromatography, Ion Exchange</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048013" MajorTopicYN="N">Clostridium thermocellum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012015" MajorTopicYN="N">Reference Standards</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AFEX</Keyword><Keyword MajorTopicYN="N">Acetylxylanesterase (Axe)</Keyword><Keyword MajorTopicYN="N">Bioreactor</Keyword><Keyword MajorTopicYN="N">GH43 hemicellulase</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>03</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>1</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24767793</ArticleId><ArticleId IdType="pii">S0960-8524(14)00437-4</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2014.03.132</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Gupta, Ashutosh" sort="Gupta, Ashutosh" uniqKey="Gupta A" first="Ashutosh" last="Gupta">Ashutosh Gupta</name></noRegion><name sortKey="Choudhary, Rajan" sort="Choudhary, Rajan" uniqKey="Choudhary R" first="Rajan" last="Choudhary">Rajan Choudhary</name><name sortKey="Das, Debasish" sort="Das, Debasish" uniqKey="Das D" first="Debasish" last="Das">Debasish Das</name><name sortKey="Das, Saprativ P" sort="Das, Saprativ P" uniqKey="Das S" first="Saprativ P" last="Das">Saprativ P. Das</name><name sortKey="Ghosh, Arabinda" sort="Ghosh, Arabinda" uniqKey="Ghosh A" first="Arabinda" last="Ghosh">Arabinda Ghosh</name><name sortKey="Goyal, Arun" sort="Goyal, Arun" uniqKey="Goyal A" first="Arun" last="Goyal">Arun Goyal</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Bioethanol production from hemicellulose rich Populus nigra involving recombinant hemicellulases from Clostridium thermocellum.
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